Fig. 3. DAAM expression in the embryonic tracheal system and the DAAM antibody. RNA in situ hybridization of DAAM (A) and immunohistochemical staining with a polyclonal anti-DAAM serum (B) revealed a strong expression in the developing embryonic tracheal system. Brown staining in A and red staining in B show DAAM expression in a stage 16 embryo. The anti-DAAM serum barely detects any DAAM protein in a DAAM^Ex68 homozygous mutant embryonic trachea (C,D) and in mitotic clones induced in DAAM^Ex68, w, FRT19A/w, arm-lacZ, FRT19A; ey-flp/+ larval eye imaginal disc [E-H; homozygous mutant clones (white arrows) lack DAAM staining]. Clones are marked by absence of ß-gal (green). Elav (blue) is a neuronal marker. H is the merge of E-G. Anterior is towards the left, and dorsal is upwards in all panels.