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Figure 2


Fig. 2. Localization of endogenous and MCK-B2 transgene-derived laminin ß2. (A,B) In control skeletal muscle, endogenous mouse laminin ß2 (A) is concentrated at synapses (arrows) doubly labeled by {alpha}-bungarotoxin (B); ß2 is also found in extrasynaptic regions of muscle fibers (A). (C,D) In MCK-B2 transgenics, antibody specific for transgene-derived rat ß2 (C) labels only synapses in skeletal muscle (arrows), identified by {alpha}-bungarotoxin (D). (E-H) Transgene-derived rat laminin ß2 is also found in cardiac muscle BMs (E), in circular (cm) but not longitudinal (lm) smooth muscle or crypt (c) epithelial BMs of intestine (G), and weakly in large airway smooth muscle of lung (arrow in H) but not in alveolar (alv) BMs. No rat ß2 was detected in glomeruli (g) in kidney (F). Scale bars: 25 µm in A-D; 100 µm in E,H; 50 µm in F,G.