Fig. 2. Synergistic effects of sor-1 and sop-2. (A-F) An earlier onset of ectopic expression of egl-5 in sop-2; sor-1 mutants. Lack of expression of egl-5::gfp in the head region in a `pretzel' stage embryo (arrow) in sor-1 (A,B) and sop-2 (C,D) mutants. Ectopic expression of egl-5::gfp in the head region in a `pretzel' stage sop-2; sor-1(RNAi) embryo (E,F) (arrow). egl-5 is expressed in the tail region in all mutant embryos. Nomarski images (A,C,E); Fluorescence images (B,D,F). (G) Expression of egl-5::gfp in the tail of a sor-1(RNAi) mutant (bracket). (H) Expression of egl-5::gfp in the tail of a sop-2 mutant (bracket). (I) Dramatically expanded expression domain of egl-5::gfp in the tail of a sop-2; sor-1(RNAi) mutant (bracket). (J) Ectopic expression of egl-5::gfp in the middle body region in a sop-2; sor-1(RNAi) mutant (marked with a bar). Irregular fluorescence particles are gut autofluorescence.