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Figure 4


Fig. 4. Analysis of BRK1p::BRK1-T7/His transgenic plants. (A) Scanning electron micrograph showing aberrant (white arrowheads) and normal (black arrowhead) trichome morphologies observed in some BRK1p::BRK1-T7/His transgenic plants. Scale bar: 100 µm. (B) Northern blot comparing BRK1 RNA levels in four BRK1p::BRK1-T7 transgenic lines with abnormal trichomes with four non-transgenic Columbia samples (blot was probed with a full-length BRK1 cDNA, which does not distinguish between endogenous and transgene mRNAs). BRK1:ß-tubulin mRNA ratios were higher in all four transgenic samples than in the wild-type samples.