Fig. 7. F-actin organization in expanding cotyledon pavement cells transiently expressing GFP-talin. (A-C) Columbia wild-type (surface area is 2220 µm² for cell in A and 5660 µm² for cell in C); (D-F) brk1-1 mutant. White arrowheads indicate local enrichments of cortical F-actin associated with apparently emerging lobes; gray arrowheads indicate enrichments that do not appear to be associated with emerging lobes. Scale bar: 13 µm. (G) Quantitative analysis of cortical F-actin distribution in wild type (n=22 cells) and brk1-1 mutant (n=36 cells). Bright actin, upper half of the fluorescence intensity range; intermediate actin, 25-50% of range; dim actin, bottom 25% of range. Error bars shown are s.e.m.