Fig. 4. Differential allocation of N-cadherin to the apical daughters of perpendicularly dividing DM progenitors. (A) Transverse section showing the DM and underlying myotome (M) co-stained with nuclear Hoechst (blue) and N-cadherin (green). Cell divisions occur at the apical portion of the DM (arrowhead). (B-E) High magnifications of dividing cells stained with Hoechst (blue), N-cadherin (green) and -tubulin to label centrosomes (red). (B) A cell dividing parallel to the mediolateral axis of the DM. Both daughter cells retain N-cadherin staining at their apical aspects. (C,D) Perpendicularly dividing DM cells in which only the apical progeny remains N-cadherin positive. (E) Oblique mitosis showing a similar segregation of N-cadherin to the apical daughter cell. In contrast to the DM, myotomal cells express homogeneous N-cadherin on their surface. Panels in second and third rows show, separately, Hoechst staining of nuclei associated with -tubulin and N-cadherin with -tubulin. n, at least 60 mitoses were scored with equal N-cadherin distribution in progeny of all parallel mitoses, and apically localized N-cadherin in all perpendicular divisions. Scale bar: 33 µm in A; 10 µm in B-E.