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Figure 5


Fig. 5. Effects of N-cadherin-mediated adhesion on cell translocation and fate segregation of DM sheet progenitors. (A,E) Control GFP-treated somites (n=31/31 embryos). (B,G-I) cN390{Delta}-GFP-treated somites (n=27/30 embryos). (C,F) Full-length N-cadherin-treated somites (n=27/30). (D) N-cadherin (CBR-)-treated somites (n=12/12). Desmin is red, GFP is green and Hoechst staining is blue in A-D. The blue products in G-I are in situ hybridizations for Alx4 (G,H) and Pax7 (I). Electroporations were performed in somite 22 of 28ss embryos (A-H). (A-D) Transverse sections two days after electroporation, showing (A) the distribution of control GFP-treated cells among both the dermis and myotome, (B) cN390{Delta}-GFP-treated cells in the dermal domain only, and (C,D) wild-type N-cadherin or N-cadherin (CBR-)-treated cells in myotome only. (E,F) Whole mounts showing that wild-type N-cadherin-treated DM cells generate fibers (F), whereas GFP only-treated cells remain mesenchymal (E). (G,H) DM cells that received cN390{Delta}-GFP relocate into the nascent dermis while maintaining expression of Alx4 (arrows). (I) Premature dissociation of DM cells induced by focal electroporation of cN390{Delta}-GFP 10 hours after electroporating an epithelial somite. Dissociating cells downregulated Pax7 mRNA (arrowheads) when compared with similar cells still resident in the epithelium (arrows) or with untransfected cells. Scale bar: in A, 40 µm; in B, 30 µm; in C,D, 50 µm; in G,H, 60 µm; in I, 15 µm.