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Figure 6


Fig. 6. cN390{Delta} causes DML dissociation and lack of myotome colonization. (A-F) Control GFP; (G-L) cN390{Delta}-GFP. Electroporations were directed to the DML of early dissociating somites 22-23 of 28ss embryos. GFP is green; desmin, red. (A-D) Five hours after transfection, labeled DML cells retain their epithelial structure as revealed in dorsal views (A,B) and transverse sections (C,D, arrowheads, n=15/17). (E) Thirty hours after electroporation, GFP-positive myofibers derived from the DML occupy the medial-most aspect of the myotome (arrowheads). The DML itself retains its epithelial structure (n=12/14). (F) Dermal stage showing a larger number of labeled DML-derived fibers in the desmin-positive myotome with a still epithelial DML. (G-J) Five hours after transfection, DML cells that received cN390{Delta}-GFP have begun dissociating, as revealed in dorsal views (G,H, arrows) and transverse sections (I,J, arrowheads, n=8/10). (K) By 30 hours, cN390{Delta}-GFP-treated cells have lost their epithelial morphology and have located subectodermally (arrows). None of them generated myofibers (n=8/11). (L) Dermal stage to further emphasize the dissociation of DML cells and the lack of myotomal colonization. Scale bars: 20 µm in C,D,I,J; 10 µm in E,F,K,L.