Fig. 6. Expression of Mbh2 by EYFP-labelled cells that form clusters in the pons at E18.5. (A-D) EYFP-labelled cells at the level of the rostral (A,B) and caudal (C,D) pons. (B,D) Mbh2 expression in locations corresponding to A and C. PGN neurons located on the contralateral side were distributed only in the dorsal PGN (A), although RTN neurons were symmetrically distributed on both sides (C). (E-J) Higher-power views of the area outlined by rectangles e-j. Although the ventral region of the ipsilateral PGN was densely occupied by labelled cells (E), the corresponding region in the contralateral PGN contained axons (F, arrowheads). Most labelled neurons on both ipsilateral (G,I) and contralateral (H,J) sides expressed Mbh2 (G-J, right). Arrows in each panel of I and J indicate identical areas. (A,C,E,F) Immunostaining for EYFP; (B,D) Mbh2 in situ hybridization signals. (G-J) Immunostaining for EYFP (green) and Mbh2 in situ hybridization pattern (purple) in the same section. EYFP was electroporated into the left lRL. E18.5 mouse transverse sections. Dorsal is upwards. Scale bars: in F, 200 µm for A-D and 50 µm for E,F; in J, 50 µm for G,H and 20 µm for I,J.