Fig. 2. Rescue of gonad compaction. Expression of indicated FOI proteins in foi-mutant embryos. (A) Graph indicates percentage of fully compacted gonads, as judged by -VAS staining (average of two independent lines +s.d.; asterisk indicates construct for which three independent insertion lines were tested). (B-G) Confocal images of representative stage 15-16 gonads immunolabeled with -VAS (germ cells, red) and -ZFH-1 (SGPs, green). (B) Wild type, (C) foi mutant, (D-G) foi mutants with the indicated HA-FOI proteins expressed. Only expression of HA-FOI (D) or Y646A (G) rescues gonad compaction. Insets in C and E represent the most severe phenotype observed for these two genotypes; the full panels represent a less severe, but more representative, phenotype. Scale bars: 10 µm.