Fig. 6. shg mRNA is reduced in foi mutants. (A-D) Confocal images of embryos labeled by fluorescent in situ hybridization to reveal shg RNA (red) and immunolabeled with -VAS (germ cells, green). (A'-D') shg RNA channel alone. shg RNA is observed in SGPs of wild-type embryos both before (A, stage 13) and after (C, stage 15) gonad coalescence, but is not detected in foi-mutant gonads at either stage (B, stage 13; D, stage 15). (E,F) Confocal images of stage 15 wild-type (E) and foi-mutant (F) gonads heterozygous for the shg^k03401 enhancer-trap immunolabeled with -ß-GAL (enhancer trap, green) and -VAS (germ cells, blue). SGPs were identified using -EYA (not shown). (E',F') Identical images showing only -ß-GAL. SGPs in wild-type gonads (E') exhibit slightly increased shg^k03401 expression compared with surrounding tissues, while SGPs in foi-mutants (F') exhibit weaker expression than surrounding tissues. The perimeter of gonad is outlined. Scale bar: 10 µm.