Fig. 7. C/EBP
does not regulate C/EBPß and C/EBP
in vivo.
C/EBP
mRNA was decreased but expression of C/EBPß and C/EBP
mRNA was maintained in lungs from Cebpa
/
mice. Total lung RNA was prepared from fetal mouse lung at E18.5. RNAse
protection assay was performed with 7 µg of total RNA for estimation of
C/EBP
, C/EBPß, C/EBP
, and ribosomal protein L32 mRNA.
Protected fragments were standardized to the internal control L32 and
normalized to control littermates.