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Figure 7


Fig. 7. C/EBP{alpha} does not regulate C/EBPß and C/EBP{delta} in vivo. C/EBP{alpha} mRNA was decreased but expression of C/EBPß and C/EBP{delta} mRNA was maintained in lungs from Cebpa{Delta}/{Delta} mice. Total lung RNA was prepared from fetal mouse lung at E18.5. RNAse protection assay was performed with 7 µg of total RNA for estimation of C/EBP{alpha}, C/EBPß, C/EBP{delta}, and ribosomal protein L32 mRNA. Protected fragments were standardized to the internal control L32 and normalized to control littermates.