Fig. 4. Developmental defects of Dnmt3b^-/- embryos. (A) Analysis of embryos derived from intercrosses of Dnmt3b^+/- mice. Numbers in parentheses indicate the number of abnormal embryos. (B) Gross morphology of Dnmt3b^-/- embryos and a wild-type littermate at 13.5 dpc. Some Dnmt3b^-/- embryos showed a smaller and paler fetal liver than their wild-type littermates, which could be recognized from outside (middle embryo, arrow). Some Dnmt3b^-/- embryos showed bleeding at the head region (right embryo, arrow). (C) Posterior view of the Dnmt3b^-/- embryo shown in B. Most of the Dnmt3b^-/- embryo showed subcutaneous edema (arrows) at 13.5 dpc. (D) Ventricular septum defect in the heart of a Dnmt3b^-/- embryo at 14.5 dpc. The right panel shows a higher magnification of the inset shown in D. (E) Ectopic hemorrhage at the dorsal root ganglion of a Dnmt3b^-/- embryo at 12.5 dpc. (F) Malformation of the fetal liver in Dnmt3b^-/- embryos at 13.5 dpc.