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Figure 2


Fig. 2. Hox gene expression in Sfrp1-/-;Sfrp2-/- embryos. (A,B) Hoxb2 expression in control (A) and Sfrp1-/-;Sfrp2-/- (B) embryos at E9.25. An arrow indicates the anterior boundary of Hoxb2 expression at rhombomere 3. ov, otic vesicle. (C,D) Hoxa7 expression in control (C) and Sfrp1-/-;Sfrp2-/- (D) embryos at E9.25. An arrow and an arrowhead indicate the anterior boundary of Hoxa7 expression in the spinal cord and somite, respectively. (E-H) Hoxd10 and myogenin gene expression in control (E,G) and Sfrp1-/-;Sfrp2-/- (F,H) embryos at E10.5. Myogenin expression was visualized by INT/BCIP in double in situ hybridization (red). An arrowhead depicts the anterior boundary of Hoxd10 in the somite. (I) Summary of the skeletal pattern and Hoxb2, Hoxa7 and Hoxd10 expression in control and Sfrp1-/-;Sfrp2-/- embryos. Correlation between vertebrae and somite number (12-17) was estimated from myogenin expression in Sfrp1-/-;Sfrp2-/- embryos (E,F). FL, forelimb; HL, hindlimb. Scale bars: 250 µm.