Fig. 4. ph mutant clones, induced before versus after the prepupal ecdysone peak, exhibit distinct phenotypes. Composite confocal images of mosaic adult brains containing clones of wild-type or ph mutant neurons that were induced at the mid-3rd instar stage (A-D) or two days after pupal formation (2D APF) (E-H). (A,B,E,F) Wild-type brains exhibit different patterns of clones with GAL4-OK107 (A,E) versus GAL4-NP225 (B,F). (C,D) Both GAL4s labeled analogous ph mutant clones following induction of mitotic recombination at the mid-3rd instar stage. (G,H) By contrast, GAL4-OK107 (G), but not GAL4-NP225 (H), permits labeling of mid-pupa-born ph mutant MB neurons. Genotype: (A,E) FRT19A/FRT19A,hs-FLP,tubP-GAL80;UAS-mCD8-GFP/+;GAL4-OK107/+; (C,G) FRT19A,ph⁶⁰²/FRT19A,hs-FLP,tubP-GAL80;UAS-mCD8-GFP/+;GAL4-OK107/+; (B,F) FRT19A/FRT19A,hs-FLP,tubP-GAL80;UAS-mCD8-GFP/GAL4-NP225; (D,H) FRT19A,ph⁶⁰²/FRT19A,hs-FLP,tubP-GAL80;UAS-mCD8-GFP/GAL4-NP225. Scale bar: 20 µm.