Fig. 4. Temperature-shift time-course experiments with temperature-sensitive alleles of FGF pathway components. (A) Synchronized egl-15(n484); trIs10 L1 hatchlings that were raised at 20°C were either kept at 20°C or shifted to 25°C as hatchlings (0 hours), or 6, 12, 18, 24 or 30 hours after hatching. The number of EMEs was counted in these animals upon reaching young adulthood. The number of EMEs in egl-15(n484); trIs10 animals raised at 25°C is also shown. A blue asterisks indicates that the number of EMEs is significantly greater than those in animals raised at 20°C (P<0.001). A black asterisks indicates that the number of EMEs is significantly different compared to animals raised at both temperatures (P<0.001). A red asterisks indicates that the number of EMEs is significantly lower compared to animals raised at 25°C (P<0.001). The number of animals counted is shown at the base of each bar. Error bars represent the s.e.m. (B) Synchronized trIs10; sos-1(cs41) hatchling L1s that were raised at 20°C were either kept at 20°C or shifted to 25°C as hatchlings (0 hours), or 6, 12, 18, 24 or 30 hours after hatching. (C) Synchronized egl-15(n484); trIs10 hatchling L1s that were raised at 25°C were either kept at 25°C or shifted to 20°C as hatchlings (0 hours), or 6, 12, 18 or 24 hours after hatching. (D) Synchronized sos-1(cs41); trIs10 hatchling L1s that were raised at 25°C were either kept at 25°C or shifted to 20°C as hatchlings (0 hours), or 6, 12, 18 or 24 hours after hatching. For B-D, the notation on the chart is the same as that in A.