Fig. 9. Enhancement of Snail2-mediated activation of the Snail2 promoter by PKA signal. Sox9, Sox9^m1m2, Snail2 or Sox9+Snail2 were transfected into NIH3T3 cells along with D0.1-Luciferase reporter. To activate PKA signal, either 1 mM of 8-bromo-cAMP (+cAMP) was added in culture or constitutively active PKA (CA-PKA) was co-transfected. Results are shown as a fold-induction compared with the result obtained from cells transfected with D0.1-Luciferase and an empty pyDF30 vector.