Fig. 6. Altered migration patterns of ventrally migrating neurons by overexpression of ephrin A5. (A) Experimental procedures for ephrin A5 misexpression in whole-embryo culture system. (B-D) Migration patterns of DiI-labeled neurons in the wild-type (B,C) and Pax6 mutant (D) embryos, in which ephrin A5 and/or GFP expression vector were electroporated. DiI-labeled neurons pass through the area in which GFP expression vector was electroporated (B,B'), whereas these neurons stop at the border area with misexpressed ephrin A5 expression vector and GFP vector (arrowheads, C-C'). (D,D') DiI-labeled neurons stop at the border area with misexpressed ephrin A5 in the Pax6 mutant embryo (arrowheads in D). Scale bars: 500 µm in D; 100 µm in D'.