Fig. 8. Altered distributions of early-born olfactory cortex neurons in the
Pax6 and ephrin A5 mutants. (A) Nissl staining of the
E18.5 rat telencephalon. (B-E) Immunostaining with anti-BrdU antibody
in E18.5 wild-type rat (B), Pax6 mutant rat (C), E18.5 ephrin
A5+/– mouse (D) and ephrin
A5–/– mouse (E) telencephalon. BrdU was
injected at E11.75 (rat) or E9.75 (mice). (B-E) LOT area of the olfactory
cortex. In the Pax6 mutant and ephrin
A5–/–, fewer BrdU-labeled cells are present in
the LOT area (arrowheads in C,E) compared with the wild type or ephrin
A5+/– (arrows in B,D). (F) The number of
BrdU-labeled cells in the LOT area of the wild-type, Pax6 mutant rats
and ephrin A5-deficient mice. BrdU-labeled cells are decreased in
Pax6 mutant rats and ephrin A5–/–
mice, compared with wild-type rats and ephrin A5+/–
mice. Data are presented as percentage of the BrdU-labeled cells in the
Pax6 mutant rat or ephrin A5–/– mouse
against the total number of labeled cells in the wild-type rat or ephrin
A5+/– mouse (mean±s.d. of four animals in
each group). **P<0.01. (G,H) Distribution
of BrdU-labeled cells in E12.5 ephrin A5+/– and
ephrin A5–/– mice, in which BrdU was injected
at E9.75. In ephrin A5–/– mice, the number of
BrdU-labeled cells was increased at the ventral part of the telencephalon
(arrowheads in H). (I-K) Distribution of BrdU-labeled cells in the
olfactory tubercle (inset in I) of wild-type rats and Pax6 mutant rat
embryos at E18.5, in which BrdU was injected at E11.75. The number of
BrdU-positive cells was increased in the Pax6 mutant olfactory
tubercle (arrowheads in K). (L) Comparison of the number of
BrdU-labeled cells in the olfactory tubercle of wild type, Pax6
mutant rats and ephrin A5-deficient mice. BrdU-labeled cells are increased in
Pax6 mutant rats and ephrin A5–/–
mice, compared with wild-type rats and ephrin A5+/–
mice. Data are presented as percentage of the BrdU-labeled cells in the
Pax6 mutant rat or ephrin A5–/– mouse
against the total number of labeled cells in the wild-type or ephrin
A5+/– mouse (mean±s.d. of three animals in
each group). *P<0.05, **P<0.01.
Scale bars: 500 µm in B,C,G; 100 µm in J. LOT, lateral olfactory tract;
Ncx, neocortex; Str, striatum.