Fig. 5. Hair cell polarity in the macula communis of the L. fluviatilis ear at stage 29. (A-D) Projections from confocal z-stacks of FITC-phalloidin (green) and anti-acetylated tubulin (red) double stains revealing the actin-rich stereocilia and the tubulin-rich kinocilia of the sensory hair cells, respectively. (A) Lateral view of whole dissected-out inner ear; regions shown in boxes are the areas shown in detail in B, C and D. (B,C) Lateral (B) and dorsal (C) views of sensory epithelium showing the actin-rich infracuticular network (icn), the short apical horseshoe of stereocilia (s), the long kinocilia (k) and the short cilia (c) on non-sensory cells. (C') Arrow, pointing from the stereociliary bundle towards the kinocilium, shows the polarity of one of the hair cells shown in C. (D) Non-sensory epithelium showing the short cilia (c) present on all non-sensory cells. (E,F) Polarity map of the anterior (E) and posterior (F) regions of the macula communis. (E',F') Stained and dissected specimen used to produce the map shown in E,F. F' is a composite of several images taken in different focal planes. Scale bars: in A, 50 µm; in B-D, 5 µm; in E,F, 25 µm.