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Figure 6


Fig. 6. PAR6ß, aPKC and ezrin lose apical cortical association in Par3{Delta}E3/{Delta}E3 epicardial progenitor cells at E10.5. (A-D) Although PAR6ß and aPKC{lambda} closely associate with the apical cortical domains in wild-type EPP cells, they show diffuse distribution in the EPP cells of Par3{Delta}E3/{Delta}E3 littermates (arrowheads). DNA was stained with DAPI (red in A'-D'). Scale bars: 10 µm. (E-J) Localization of PAR6ß, {alpha}4 integrin, ZO1, aPKC{lambda}/{zeta}, ß1 integrin, ezrin and PAR3 in cryosections of the EPP cells in wild-type and Par3{Delta}E3/{Delta}E3 littermates at E10.5. Merged images are shown in the fourth column: PAR-6ß, aPKC{lambda}/{zeta} or ezrin, red; {alpha}4 or ß1 integrin, green; ZO1 or PAR3, blue. The apical cortical association of PAR6ß and aPKC{lambda}/{zeta} in wild-type EPP cells is disturbed with the proteins diffusely distributed in the cytoplasm in Par3{Delta}E3/{Delta}E3 EPP cells (E-H, white arrowheads). However, both the wild-type and Par3{Delta}E3/{Delta}E3 EPP cells show the accumulation of {alpha}4 and ß1 integrins in the basal subdomains (E-J, arrows), and ZO1 concentrates immediately apical to {alpha}4 or ß1 integrins (E-H, black arrowheads). The apical cortical association of ezrin in wild-type EPP cells is mostly lost in Par3{Delta}E3/{Delta}E3 EPP cells (I,J, arrowheads). The asterisk in J indicates an aberrant accumulation of Par3{Delta}E3/{Delta}E3 EPP cells in the proepicardial serosa. Scale bars: 10 µm.