Fig. 7. In situ hybridization analysis of Wnt1, Otx2 and T. The expression of these markers was relatively unaffected in Mll^–/– embryos (B,D-F). Expression of Wnt1 in wild-type E9.5 embryo (A) compared with Mll^–/– E10.5 (B). Buckling of the neural tube is caused by the loss of somites. Expression of Otx2 in wild-type E9.5 embryo (C) compared with Mll^–/– E10.5 embryo (D). Expression of brachyury (T) in Mll^–/– embryos at E8.5 (E) and E9.5 (F) shows that it was correctly established and maintained.