Fig. 6. Reduction of interneurons in the olfactory bulb of Fez-deficient mice. (A-D) Neurons born at E14.5 (A,B) or E16.5 (C,D) in wild-type (WT, A,C) and Fez-deficient (Fez^–/–, B,D) embryos were labeled with bromodeoxyuridine (BrdU) for two hours, and the proliferating neuronal cells analyzed the same day. Coronal sections of the olfactory bulb were stained with anti-BrdU antibody and DAB, and counter-stained with Hematoxylin; medial is to the right. The BrdU-positive local-circuit progenitors in the VZ/SVZ of the olfactory bulb appeared normal in Fez-deficient embryos. The number of neurons born at E16.5 was reduced in Fez-deficient embryos compared with wild type (55.5±7.6%). BrdU-positive cells from five sections were counted (n=3).