Fig. 7. Defects in the rostral migratory stream (RMS) in Fez-deficient mice. (A,B) Neurons born at E14.5 in control (WT) and Fez-deficient (Fez^–/–) embryos were labeled with BrdU and their positions were determined at E18.5; coronal sections, medial to the right. The total number of BrdU-positive neurons in Fez-deficient embryos was slightly reduced, and they were not localized to the glomerular layer but were located mainly on the medial side of the granule cell layer. (C-F) Expression of Dlx1 at E18.5 (C,D) and E15.5 (E,F); sagittal sections, with anterior to the right. Dlx1 is expressed in interneuron progenitors of the OB and marks the rostral migratory stream (RMS, arrows). The RMS from the ganglionic eminence to the olfactory bulb was shorter in Fez-deficient embryos than in wild type at E15.5 and E18.5. The distribution of Dlx1-expressing cells was affected in the Fez^–/– olfactory bulb (arrowheads, C,D). (G,H) The expression of Slit1 in the septum and ganglionic eminence was not significantly affected in Fez-deficient embryos (H), compared with wild type (G).