Fig. 3. eve is necessary for grain expression and for Hb9 repression in both aCC and RP2 motoneurons. Stage 12 (A-D) or stage 15 (F-I) eve^RP2A/+ heterozygote (eve mosaic/+) (A,C,F,H) and eve^RP2A homozygote mutant (eve mosaic) (B,D,G,I) embryos. Arrows and arrowheads indicate aCC and RP2, respectively (visualized using RN2-GAL4/UAS-lacZ). (A,C,F,H) eve mosaic/+ RN2-GAL4/UAS-lacZ showing that Grn is expressed in aCC and RP2 at stage 12 and stage 15, while Hb9 is not. (B,D) In stage 12 eve mosaic mutant, Hb9 is derepressed in aCC and RP2 while Grn expression is not detectable in aCC but maintained in RP2. (G,I) In stage 15 eve mosaic mutants, Hb9 remains derepressed in aCC and partly in RP2, while Grn expression is not detectable in aCC but maintained in RP2. At this stage, Grn expression in RP2 appears even stronger in eve mosaic compared with wild type. (E,J) Quantification of these phenotypes.