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Figure 5


Fig. 5. In grain mutants, loss of Zfh1 expression is restricted to the aCC motoneuron. Stage 15 wild-type (A,D,G), grn mutant (B,E,H), zfh1 mutant (C,F) and grn rescue (I) (using RN2-GAL4/UAS-grn; grn-/-) embryos stained for Eve and pMad (A-C), Eve and Zfh1 (D-F) or Grn and Zfh1 (I). (G,H) RN2-GAL4/UAS-mEGFPF embryo stained with {alpha}-Hb9. (A-C) pMad staining in grn and zfh1 mutants appears unaffected within aCC and RP2. (D-F) In grn mutants, Zfh1 expression is not detectable in the aCC motoneuron, but RP2 maintains Zfh1 expression. Grn expression is not affected in aCC or RP2 in zfh1 mutants. (G,H) Hb9 expression is unaffected in grn mutants. (I) In grn rescue experiments, Zfh1 expression is restored in aCC showing the cell autonomous effect of grn on Zfh1 expression in this motoneuron. Arrowheads and asterisks indicate aCC and RP2, respectively.