Fig. 2. Defects in dorsoventral patterning caused by loss of ik2 in the female germline. (A-C) Dark-field views of larval cuticle preparations at the end of embryogenesis from (A) wild type, (B) a weakly ventralized embryo from an ik2^alice germline clone female and (C) a ventralized embryo from an ik2¹ germline clone female. (D-F) Eggshells produced by (D) wild type, (E) an ik2^alice germline clone female and (F) an ik2¹ germline clone female. Wild-type eggshells have two dorsal chorionic appendages, whereas the eggs in E and F have a single or no dorsal appendages, respectively. (G-I) Twist expression. Twist is expressed in the ventral 25% of cells of the wild-type blastoderm embryo (G), but is expressed in an expanded domain in embryos produced by ik2¹ germline clone females (H,I). Anterior to the left; dorsal is up in G-I.