Fig. 3. Defects in mRNA localization and expression during oogenesis caused by loss of ik2 in the female germline. (A-C) Fluorescent in situ hybridization shows that osk mRNA is localized to the posterior pole of a stage 9 wild-type oocyte (A), but is also found at the lateral cortex of an ik2^alice oocyte (B), and at the anterior and lateral cortex of an ik2¹ oocyte (C). (D-F) In situ hybridization shows that grk mRNA is localized to the dorsoanterior corner of the wild-type oocyte at stage 8-9 (D), but is also found along the anterior border of an ik2^alice oocyte (E), and is not enriched dorsally in an ik2¹ oocyte (F). (G-I) pipe mRNA is expressed in the follicle cells on the ventral side of the wild-type stage 10 egg chamber (G), but can also be detected in dorsal follicle cells of an ik2^alice egg chamber (H), and is expressed at high levels both ventrally and dorsally in an ik2¹ egg chamber (I). pipe and grk in situ hybridizations were performed in parallel with wild-type controls, and all samples were developed for equal lengths of time. Anterior to the left, dorsal up.