Fig. 7. Ectopic F-actin colocalizes with ectopic Gurken protein in ik2 mutant oocytes. (A,B) Grk protein (red) is localized in the dorsoanterior region of the wild-type stage 10 oocyte (A), but is found at the anterior of the ik2¹ oocyte (B). (C-E) Grk (red; C) colocalizes with F-actin, visualized by phalloidin staining (green; D) in a wild-type stage 8/9 egg chamber; a merged image is shown in E. (F-H) In some ik2¹ germline clones, Grk (red) is present in aggregates (F) that colocalize with F-actin (green; G); a merged image is shown in H. Nuclei are stained with DAPI (blue). Anterior to the left, dorsal up.