Fig. 8. In vivo accumulation of macrophages in the vascular wall after arterial occlusion. (A,B) Post-mortem angiography shows the right femoral artery of a rat prior to (A) and 7 days after (B) its occlusion. The prominent appearance of collaterals (B, arrows) after femoral artery occlusion is seen, as reported earlier (Ito et al., 1997). (C,D) Immunofluorescence studies on sections from rat collateral arteries show KDR staining in endothelial cells (C) and the intramural accumulation of macrophages (ED2-antibody, red staining) 3 days after occlusion (D). No notable BrdU incorporation (E) is detectable in mature immunoselected macrophages from the wall of collateral arteries after femoral artery occlusion, indicating that these cells do not proliferate. CD133⁺ cells isolated from human mobilized peripheral blood and used as positive control show an extensive BrdU incorporation (F). DAPI staining was used to visualize all cells.