Fig. 6. Expression of NOTCH pathway genes and Edar in wild-type and p63 mutant embryos. (A,C,E,G) Notch1, Notch2 and Notch3 were co-expressed in dental and oral epithelium in wild-type suprabasal cells. (B,D) Notch1 transcripts were absent in p63 null epithelium at E11 and E12. (F,H) Notch2 and Notch3 showed normal expression in the p63 mutants. (I) At E12, Jag1 was intensely expressed in suprabasal cells in the epithelium and in the mesenchyme surrounding the tooth bud in wild-type embryo. (J) The p63 mutant epithelium was devoid of Jag1 transcripts but faint expression was seen in the mesenchyme. (K,L) Jag2 was expressed in the oral epithelium in both wild-type and mutant embryos. (M,N) In E12 skin epithelium, intense Notch1 expression was seen in the wild-type embryo, whereas transcripts were not detected in the mutant littermate. (O,P) Jag1 was not observed in p63-deficient skin epithelium. (Q-T) The intensity of Edar expression was weaker in the oral, dental and skin epithelium of p63 mutants than in wild-type embryos. Scale bars: in A, 200 µm for A,B; in C, 500 µm for C-L; in M, 200 µm for M-T.