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Figure 1


Fig. 1. Expression of Oct4 and outgrowth of NT blastocysts. (A,C,E) In vivo fertilised control embryos. (B,D,F) ES-cell NT blastocysts. (A,B) Whole-mount in situ hybridisation with Oct4 mRNA probe. (C,D) Immunofluorescent staining using an Oct4 antibody. Overlapping of chromatin staining (red) and Oct4 expression (green) is revealed by a yellow stain. Both Oct4 mRNA and proteins are correctly expressed in ICM of NT embryos. (E,F) Examples of 3-day cultures of control and NT blastocysts. (G) Mean surface occupied by the ICM-derived clusters of cells (arbitrary units). No statistical difference could be detected between outgrowths.