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Figure 5


Fig. 5. Molecular characterisation of NT embryos with abnormal morphologies. Whole-mount in situ hybridisation showing the expression of brachyury (A-D), Nodal (E,F), Bmp4 (G,H), Eomes (I,J) and Cdx2 (K,L) in primitive-streak stage (A,B,E-L) and neural-plate stage (C,D) embryos. (A,C,E,G,I,K) Controls; (B,F,J) NT embryos with abnormal shape; (D,H) NT embryos displaying the `large Exe' phenotype. Scale bars: in A, 100 µm for A,B, 50 µm for C,D; in E, 100 µm for E-L.