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Figure 4


Fig. 4. Expression changes in the Hes and Hesr gene families at E10.5. (A) Semi-quantitative RT-PCR analysis showing upregulation of Hesr1, Hes1 and Hes5 in NICD1-activated hearts. Hesr1 is ectopically induced at particularly high levels in the ventricle. In situ hybridization analysis also revealed the ectopic induction of Hesr1 in the ventricle and AV canal of NICD1-activated hearts (C), whereas Hesr1 is expressed only in the atrium in wild-type hearts (B). In contrast to Hesr1, Hesr2 is expressed in the ventricle in wild-type hearts (D) and its distribution is not altered in NICD1-activated hearts (E). Abbreviations: LA, left atrium; RA, right atrium; LV, left ventricle; RA, right ventricle. Scale bar: 500 µm in E.