Fig. 2. The nub-1 insertion allele. (A) Diagram of the nub-1 T-DNA insertion allele. (B) Expression of NUB and TUBULIN (TUB) detected by reverse transcription followed by polymerase chain reaction (RT-PCR), using RNA isolated from Col-0 and jag nub inflorescence tissue. (C,D) Phenotypes observed in AP1::NUB-RNAi jag-1 transgenic plants. (C) The valve tissues are often shredded near the apical region of the gynoecium, resulting in exposure of the ovules. (D) Microsporangia development is absent in the anther (compare with Fig. 3D). ov, ovule. Scale bars: 200 µm in C; 50 µm in D.