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Figure 1


Fig. 1. T-DNA insertion alleles of LMI1. (A) Map of the LMI1 locus. Exons are shown as rectangles, introns as black lines. The homeodomain is indicated in red, the adjacent leucine zipper motif in blue. The four SALK insertion lines analyzed are indicated as triangles. The distance between the lines connecting each insertion with the sequence indicates the size of the deletion in each insertion. The lmi1 allele designation and SALK number are indicated. (B) Semi-quantitative RT-PCR analysis of LMI1 expression in four-day-old seedlings of the insertion lines compared with wild type (Col). PCR was performed with primers flanking the insertions (LMI1; primers L5' and L3' in A) or upstream of the lmi1-1 insertion (LMI1up; primers Lu5' and Lu3' in A). The eukaryotic translation initiation factor 4A (EIF4) was amplified as an internal control.