Fig. 4. Delayed AP1 induction and reduced CAL expression in lmi1-1 lfy-10. (A) Quantitative real-time RT-PCR analysis of the expression of the known direct LFY targets AP1, CAL, and LMI1 through LMI5 in eleven-day-old lmi1-1 lfy-10 compared with lfy-10. The values were normalized using eukaryotic translation initiation factor 4A (EIF4). Expression levels in lfy-10 lmi1-1 were set to one to facilitate comparison of the expression of the different genes. The mean and standard error of three technical replicates is shown. Similar results were obtained using an independent biological replicate (not shown). (B) Quantitative real-time PCR amplification of CAL and AP1. For CAL expression, lmi1-1 lfy-10, lmi1-4 lfy-10 and lfy-10 seedlings were assayed at 7, 9, 11 or 13 days of age. For AP1 expression, lmi1-1 lfy-10 and lfy-10 seedlings were assayed at 9, 11 or 13 days of age. Similar results were obtained for both CAL and AP1 using an independent biological replicate (not shown). Normalization was as in A. (C) Transverse sections through the inflorescence meristems of fixed fifteen-day-old lfy-10 (top) and lmi1-1 lfy-10 (bottom) seedlings. Flowers (F) and secondary inflorescences (I) are indicated. Scale bars: 100 µm.