Fig. 1. A requirement for ac and sc in AWM mechanosensory organ development after pI selection. (A-E) ac, sc and sens regulate the number of AWM stout bristles. Close-up views of the AWM of (A) Canton-S (wild type, wt), (B) sc^10-1/Y, (C) sc^10-1/Y; sens^E2/+ and (D) sc^10-1/Y; src/+ are shown. src, sens genomic rescue construct; these flies have three copies of the wild-type sens gene. Arrow and arrowhead in A point to a chemosensory bristle and a stout bristle, respectively. (E) Quantification of the number of stout bristles in A-D; 10-14 wings were quantified for each genotype. One-way ANOVA with Scheffe error protection indicates that the difference between sc^10-1/Y and sc^10-1/Y; src/+ is not statistically significant. Moreover, a t-test for independent samples shows that sc^10-1/Y (^*) and sc^10-1/Y; sens^E2/+ (^**) are significantly different from all of the other genotypes (P<0.0001). Error bars indicate s.e.m. (F-F'') Double-staining of the AWM of an 8- to 10-hour APF A101-lacZ pupa for ß-Gal (green) and Sens (red) indicates colocalization of the two proteins in the mechanosensory pIs and the internal cells of the presumptive chemosensory organs (asterisks in F). (G-I) Sens staining of the AWM of (G) A101-lacZ (wild type), (H) sc^10-1/Y and (I) sc^10-1/Y; sens^E2/+ pupae at 12-14 hours APF does not show a significant difference in the number of mechanosensory pIs between these genotypes. Note the absence of chemosensory clusters in H and I.