Fig. 4. Da and Sens synergize in vitro and in vivo, and bind to each other. (A-D) Co-expression of Da and Sens in the wing results in a synergistic increase in the number of ectopic SOs generated. The third wing vein region of (A) sca^109-68-GAL4/UAS-da⁵², (B) sca^109-68-GAL4 UAS-sens^C12 and (C) sca^109-68-GAL4 UAS-sens^C12/UAS-da⁵² flies are shown. In D, the number of extra SOs are quantified for each genotype (n17). Arrows in insets indicate the extra campaniform (dome-shaped) sensilla. (E) Sens strongly increases the level of ac-luciferase transcription induced by Da. (F) In vitro-translated Sens can be pulled-down using GST-Da, but not with GST alone. Western blotting with an anti-Sens antibody was used for detection.