Fig. 6. Da is able to induce pI formation in the absence of Sens function. (A) A MARCM da³ clone that overexpresses sens using tub-GAL4. Note the absence of microchaetae in the mutant clone (the closed line in the close-up view), which is marked by crinkled. (B,C) Low (B) and high (C) magnification views of two MARCM clones of sens^E2 that overexpress da using tub-GAL4. No microchaetae are formed in the clone, which is marked by mwh. (D-D'') Overexpression of da in a MARCM sens clone in the thorax using tub-GAL4 results in the generation of numerous additional Elav⁺ cells (red), often clustered. Note that the neurons are usually well spaced in the wild-type tissue. The image is from a 36-hour APF pupa. GFP (blue) marks the nuclei of the sens mutant cells.