Fig. 1. MSI1 is an activator of floral transition. (A) 35-day-old wild-type (WT) and msi1-tap1 plants grown in long days (LD). Note, msi1-tap represents msi1-1/msi1-1 P_MSI1::MSI1-TAP. (B) Analysis of the flowering time of four independent msi1 lines complemented with a TAP-tagged MSI1 protein grown in LD. (C) Analysis of the flowering time of three independent msi1 lines complemented with untagged MSI1 protein grown in LD. Note, msi1-notap represents msi1-1/msi1-1 P_MSI1::MSI1. (D) Flowering time of msi1-tap1 grown in LD presented in number of days to bolting. (E) Analysis of the flowering time of the msi1-tap1 line complemented with a 35S::MSI1 construct, and of MSI1 antisense (msi1-as) plants grown in LD. (F) Analysis of the flowering time of wild-type plants carrying the 35S::MSI1 construct grown in continuous light. Graphs show means±s.e.m. of total rosette leaves at bolting (B,C,E,F) or days to bolting (D).