Fig. 4. Expression of the floral activator SOC1 is delayed in msi1-tap1, msi1-as and msi1-notap3. (A) Expression analysis of SOC1 in wild-type and msi1-tap1 during seedling development in LD. Plants were grown for 5 to 11 days after germination (DAG) on plates containing MS medium in LD. RNA was extracted from total seedlings grown on plates containing MS medium in LD and harvested every second day at 4 hours after start of the light period. (B) Quantification of the results in A. Values were normalized to the corresponding GAPDH control. The maximal value in wild-type was set to 1.0. Black symbols, wild type; white symbols, msi1-tap1. (C) Expression analysis of SOC1 in msi1-tap1 in SD. RNA was extracted from total 15- and 18-day-old seedlings grown on plates containing MS medium in SD harvested at 1 hour before the end of the light period. (D) Expression analysis of SOC1 in msi1-as and msi1-notap3 in SD. RNA was extracted from total 14-day-old seedlings grown on plates containing MS medium in SD harvested at 1 hour before the end of the light period. (E) MSI1 expression in the shoot apex. RNA was isolated from dissected 18-day-old seedlings grown in SD. STM was used an apex-specific control. GAPDH was used as control in A,C-E. n.t., no template control.