Fig. 6. Altered histone methylation and acetylation at the SOC1 locus in msi1-tap1 seedlings. (A) Scheme of the transcribed region of the SOC1 locus and the fragments used for chromatin immunoprecipitation (ChIP). The gray and black boxes symbolize exons and introns, respectively, and the dark gray boxes, the untranslated exons. (B) Expression of the phosphofructokinase (PFK) gene At4g04040 is not changed in msi1-tap1 plants. RNA was extracted from total 15-day-old seedlings grown on plates containing MS medium in SD. (C) Quantification of PCR products after ChIP with anti-dimethyl-histone H3K4 and anti-acetyl-histone H3K9 antiserum. Values were normalized to PFK and are shown as relative enrichments in samples from msi1-tap1 versus wild-type seedlings. The gray and hatched bars represent the results of two independent ChIP experiments. Chromatin was extracted from 15-day-old seedlings grown in SD harvested at 1 hour before the end of the light period.