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Figure 3


Fig. 3. Analysis of the multimerised 22-mer lacZ construct. (A) Structure of the 4x22-mer lacZ construct. A fourfold repeated version of a 22-mer sequence, derived from within the 30-mer (Fig. 2A), was synthesised as a single oligonucleotide with BamHI cohesive ends. This was cloned into the vector Actin15{Delta}Bam:gal, which provides basal transcription sequences. (B) Expression patterns for the 4x22-mer lacZ construct. Stable transformant cells of the 4x22-mer lacZ construct in Ax2 were developed to the indicated stages of development and stained for ß-galactosidase activity. (C,D) Analysis of DIF inducibility of the 4x22-mer lacZ construct in Ax2 and dimA– cells. Stable transformants of the 4x22-mer:lacZ construct in Ax2 and in the DIF insensitive, dimA-null strain were rendered DIF-competent and then either induced or not induced with DIF. The lacZ expression level for Ax2 is presented as OD575 (C) and as raw data (D), with duplicate micro-titre wells displayed for each experimental point.