Fig. 1. Loss of ß1 integrin in mutant ENCCs and expression of other ß integrins. ENCCs, or neurons and their precursors, are detected with p75^NTR and HuD, respectively. (A-H) Frozen sections of control and mutant guts stained with anti-ß1 integrin (green) and anti-p75^NTR (red) antibodies. (A-D) At E9.5, the ß1 integrin loss is mostly complete in mutant p75^NTR+ cells, even though a few of them still express small amounts of ß1 at their surface (D, arrowheads). (E-H) At E11.5, mutant p75^NTR+ cells have completely lost the ß1 integrin subunit. (I-P) Frozen sections of E13.5 control and mutant midguts stained for ß5 integrin (green) and HuD (red) (I-L), or for ß3 integrin (green) and p75^NTR+ (red) (M-P). Control and mutant ENCCs express high levels of ß5 integrin and small amounts of ß3 integrin. Scale bar: 50 µm.