Fig. 5. Neuronal differentiation of ENCCs. (A,B) Schematic representation of the E12.5 X-Gal stained guts which were immunostained with anti-NF160 and anti-HuD antibodies, recognising neuronal processes and neuron cell bodies, respectively. The regions which are colonised by ENCCs are stained in blue. The red boxes indicate the regions where pictures C-F were taken. (C,D) Distal midgut. (C) Control neurons and non-labelled ß-gal⁺ ENCCs form a scattered network occupying all the available space. (D) Mutant neurons and non-labelled ß-gal⁺ ENCCs form bundles around fasciculated neuronal processes, surrounded by ENCC-free areas indicated by asterisks. (E,F) Migratory front. Black arrowheads indicate NF160⁺ processes associated with the leading ENCCs, in control and mutant.