Fig. 6. The mutant ENCCs show a migration defect in a 3D tissue environment. (A) Schematic representation of the graft experiment protocol. Segments of control or mutant distal midgut were grafted onto segments of wild type hindguts at E12.5. (B,C) X-Gal staining of the explants after 3 days in culture. The black line represents the limit between the wild-type hindguts and the control or mutant fragments which are grafted onto them. Each red arrowhead indicates the position of the most-caudal ß-gal⁺ cell in the wild-type hindgut. Mutant ENCCs migrated less far than control ENCCs in wild-type hindguts. (D,E) Quantification of the migration defect. (D) For each of the five independent experiments, noted e1 to e5, the control explant in which ENCCs had migrated the furthest was chosen as the reference (100). The distances travelled by ENCCs in the other explants of the same litter were expressed as percentages of this maximal distance. (E) Histogram showing the average distances covered in wild-type hindguts. The average distance covered by mutant ENCCs is significantly reduced compared with the control (^*P<0.002).