Fig. 8. The ENCC migratory response to GDNF is ß1 integrin dependent. Segments of E13.5 proximal midgut were embedded in 3D collagen matrix and cultured in the presence or absence of GDNF. After 2 days of culture, neuron cell bodies were detected with HuD (red), neuronal processes with NF160 (red), glial cells with B-FABP (green) and nuclei with DAPI. (A,B,E,F) Without GDNF, no ENCCs migrated out of the explants. (C,D) In the presence of GDNF, a large number of neurons (red arrows) and glial cells (green arrows) emigrated from control explants. Neurons and glial cells were the only cell types found out of the explants, as shown by the DAPI staining. (G,H) In the mutant, no neuron cell bodies or glial cell entered the collagen matrix in the presence of GDNF, but neuronal processes did (white arrows).