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Figure 2


Fig. 2. Loss of aPKC{lambda} disrupts neuroepithelial pseudostratified structure. Coronal sections of telencephalon in control embryos (A-D) and aPKC{lambda} cKO embryos (E-H) were stained using Hematoxylin and Eosin (A-C,E-G), with anti-ßIII tubulin antibody (TuJ1, green) and Ki67 (red) (D,H), or with TuJ1 (green) and DAPI (magenta) (D',H'; arrow indicates TuJ1 staining surrounding and Ki67-negative differentiated neuron). Brains were dissected at E15.5 (A,E) or 16.5 (B-D,F-H). Higher magnification view of boxed areas in B and F are shown in C and G. aPKC{lambda} cKO embryos display abnormal protrusions in the ganglionic eminence (arrowheads in F) and lack the anterior horn of the lateral ventricle (arrow in F). Tuj1-positive cells locate at the ventricular surface (arrows in H). Scale bars: 100 µm in A,C,E,G; 1 µm in B,F; 10 µm in D,H.