Fig. 5. Loss of aPKC results in disappearance of neuroepithelial adherens junctions. (A-D) Immunofluorescence for aPKC (red), ß-catenin (green) and nuclei (blue) on confocal sections of the neocortical region in control (A,C) and aPKC cKO embryos (B,D) at E15.5. Immunofluorescence of ß-catenin alone is shown in C and D. Dot-like signals of ß-catenin are constantly seen in control embryos (A,C; arrowheads), but are rare in aPKC cKO embryos (B,D; arrow). (E-H) Electron micrographs of the ventricular surface of neuroepithelium in E15.5 aPKC cKO embryos. (G,H) High-magnification views. Adherens junctions (electron dense lines indicated by arrowheads) are constantly observed in the caudal neocortical region where aPKC is still retained (E,G), while only fragmented adherens junctions (arrow) are rarely observed in the rostral region where aPKC is predominantly lost (F,H). LV, lateral ventricle. Scale bars: 10 µm in A,B; 1 µm in E,F.